Tryptophan Hydroxylase 1 anticorps

N° du produit ABIN361412

L’anticorps Mouton Polyclonal anti-Tryptophan Hydroxylase 1 a été validé pour WB et IHC. Il convient pour détecter Tryptophan Hydroxylase 1 dans des échantillons de Lapin.

Aperçu rapide pour Tryptophan Hydroxylase 1 anticorps (ABIN361412)

Antigène: Tryptophan Hydroxylase 1 (TPH1)

Reactivité: Lapin

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Tryptophan Hydroxylase 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-Tryptophan Hydroxylase 1 anticorps

Specificité: Specific for the ~55k tryptophan hydroxylase protein.

Réactivité croisée: Humain, Rat (Rattus)

Homologie: wide variety mammalian

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 IHC (frozen sections, Haycock et al., 2002): 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Détails sur Tryptophan Hydroxylase 1

Antigène: Tryptophan Hydroxylase 1 (TPH1)

Autre désignation: TPH1

Sujet: Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001). Anti-Tryptophan Hydroxylase Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.

Poids moléculaire: '55 kDa

ID gène: 100101558

UniProt: P17290

Pathways: Feeding Behaviour